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. Author manuscript; available in PMC: 2020 Aug 28.
Published in final edited form as: ACS Appl Nano Mater. 2018 Sep 14;1(10):5389–5395. doi: 10.1021/acsanm.8b01380

Figure 5.

Figure 5.

(a) UV/vis spectra. (b) Photographs of Fe3O4 nanosheet catalysis using TMB as the substrate in the presence of H2O2 (pH 4.5 and 30 °C). (c) pH and (d) temperature dependences of the peroxidase-like activity of the Fe3O4 nanosheets and Fe3O4 NPs. The velocity of the reaction was measured using Fe3O4 NPs and Fe3O4 nanosheets. Fe3O4 nanosheets and Fe3O4 NPs show optimal pH and temperature of 4.5 and 30 °C, respectively. The maximum point in each curve was set as 100%. (e) Plot of the concentration of H2O2 at 530 μM and TMB concentration varied. (f) Plot of the concentration of TMB at 800 μM and H2O2 concentration varied. (g and h) Double-reciprocal plots with the concentration of one substrate (H2O2 or TMB) fixed and the other varied. Experimental conditions: 0.2 M sodium acetate (NaAc) buffer, pH 4.5, 30 °C.