(A and B) Post mortem in situ hybridization using specific riboprobes to assess KLF9 mRNA expression in the anterior DG (red outline) of male (A) and female (B) human subjects with recent history of stressful life events and major depressive disorder (MDD). Representative images for six, six, five, and five independent subjects per group. Scale bar: 50 μm.
(C) Quantifications of KLF9 mRNA expression (percentage background) in the anterior DG of male and female subjects. Data (mean ± SEM; n = 6 and 6 subjects per group) were analyzed using unpaired two-tailed Student’s t test. Data (mean ± SEM; n = 5 and 5 subjects per group) were analyzed using unpaired two-tailed Student’s t test; *p < 0.05, MDD versus controls.
(D) Quantifications of KLF9 mRNA expression (percentage background) in the anterior DG correlated with the severity of recent stressful life events. Data (n = 10 and 12 subjects per group). Note the significant positive correlation between the KLF9 mRNA levels and the severity of recent stressful life events in female (r2 = 0.818, p = 0.0003) but not male (r2 = 0.095, p = 0.328) subjects.
See also Table S1.