FIGURE 4.

pH dependent Esculin uptake in yeast SEY6210 cells. The cells were transformed with either empty pDR196 (Empty) or with pDR196::PvSUT1.1 (Bean) and incubated in 1 mM esculin solution with either (A) varying pH levels or (B) with the protonophore CCCP (100M) for one hour at 28°C. Box plots represent relative fluorescence values normalized for cell density (n = 3‐4). Solid black squares represent the mean while the horizontal line inside the box represent the median. Numbers above the boxes indicate percent increase compared to the empty vector control. Asterisk represent significant difference at α = 0.05 level while plus symbol (+) represent difference at α=0.10 level. NS = no significant difference versus empty vector control