Fig. 3. Impact of fibroblast growth factor 21 (FGF21) on diabetes-induced renal inflammation. Renal expression of tumor necrosis factor α (TNF-α) (A, B), CD68 (A, C), and intercellular adhesion molecule-1 (ICAM) (A, D) was tested by Western blotting assay (A). Renal expression of CD68 and CD3 was examined by immunohistochemical staining (E–H) (×400). Data are presented as mean±standard deviation (Friend virus B NIH Jackson [FVB], n=9; diabetes mellitus [DM], n=10; FGF21-treated diabetic mice [FGF21], n=6). ^aP≤0.05 for each DM vs. FVB groups, ^bP≤0.05 for DM/FGF21 vs. DM groups.