Fig. 4. Impact of fibroblast growth factor 21 (FGF21) on diabetes-induced renal apoptosis. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining (green), nuclei staining with 4′,6-diamidino-2-phenylindole (DAPI) (blue), and merge (A, B) (×400). Red arrows: apoptosis in tubule; purple arrows: apoptosis in glomeruli. BCL-2-associated X protein (BAX) and BCL-2 were examined by Western blotting assay (C, D). Data are presented as mean±standard deviation (Friend virus B NIH Jackson [FVB], n=9; diabetes mellitus [DM], n=10; FGF21-treated diabetic mice [FGF21], n=6). aP≤0.05 for each DM vs. FVB groups, bP≤0.05 for DM/FGF21 vs. DM groups.
