Fig. 5. Impact of fibroblast growth factor 21 (FGF21) on diabetes-induced renal oxidative stress. The lipid peroxide concentrations of plasma (A) and kidney (B) were measured by detecting thiobarbituric acid (TBA) reactivity reflected by the amount of malondialdehyde (MDA) formed during acid hydrolysis of the lipid peroxide compound. Renal expression of 3-nitrotyrosine (3-NT) (D), 4 hydroxynonenal (4-HNE) (E), heme oxygenase-1 (HO-1) (F), NAD(P)H dehydrogenase [quinone]-1 (NQO-1) (G), catalse (CAT) (H) and superoxide dismutase 2 (SOD2) (I) were tested by Western blotting assay (C). Data are presented as mean±standard deviation (Friend virus B NIH Jackson [FVB], n=9; diabetes mellitus [DM], n=10; FGF21-treated diabetic mice [FGF21], n=6). OVE, FVB (Cg)-Tg (Cryaa-Tag, Ins2-CALM1) 26OVE/PneJ (OVE26). ^aP≤0.05 for each DM vs. FVB groups, ^bP≤0.05 for DM/FGF21 vs. DM groups.