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. 2020 Aug 27;2(3):zcaa017. doi: 10.1093/narcan/zcaa017

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© The Author(s) 2020. Published by Oxford University Press on behalf of NAR Cancer.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — POLQ performs MH-mediated end joining and insertion in distal EJ. (A) Distal end-joining products isolated from GFP-positive cells were characterized by target site amplification and high-throughput sequencing (NGS). The percentage of end-joining products with no mutation (B) and different length MH (C). The percentage of different length of insertion (D), different types of insertion (E), insertions initiated with MH (F), insertions ended with MH (G) and insertions initiated and ended with MH (H). Unpaired t-tests were performed for statistical analysis (ns denotes P > 0.05; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001).