Table 1.
The highest proportion of read counts obtained with each cycle threshold.
PCR Cycle Number | aHighest read count (%) | bRead count (±SEM) |
---|---|---|
25 | 0/24 (0.0) | 2783 (1410) |
30 | 6/24 (25.0) | 20838 (6995) |
35 | 4/24 (16.7) | 17042 (3242) |
40 | 14/24 (58.3) | 49704 (20776) |
The proportion of samples with the highest read count for a given cycle number.
The average read count for each PCR cycle threshold (± standard error of the mean).