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. 2020 Aug 27;8(2):e001111. doi: 10.1136/jitc-2020-001111

Table 2.

Table of germ line and differentiation antigens from the Cancer Antigenic Peptide Database

Gene/protein Tumor type HLA restriction element Peptide sequence Reference Total score mutant MHC IC50 (nM) mutant
Germ-line epitopes
BAGE-1 M Cw16 AARAVFLAL Boel, 1995 −0.53 419.6
CT37/FMR1NB LC A2 YLCSGSSYFV Flores-Villanueva, 2018 0.3 8.5
Cyclin-A1 AML A2 SLIAAAAFCLA Ochsenreither, 2012 −2.08 894.4
Cyclin-A1 AML A2 FLDRFLSCM Ochsenreither, 2012 −0.46 29
GAGE-1,2,8 M Cw6 YRPRPRRY Van den Eynde, 1995 −0.26 1140.7
GAGE-3,4,5,6,7 M A29 YYWPRPRRY De Backer, 1999 1.65 13.1
GnTV M A2 VLPDVFIRC(V) Guilloux, 1996 −1.41 438.5
HERV-E RCC A11 ATFLGSLTWK Takahashi, 2008 0.21 7.8
HERV-K-MEL M A2 MLAVISCAV Schiavetti, 2002 0.43 6.9
KK-LC-1 LC B15 RQKRILVNL Fukuyama, 2006 −0.6 517.9
KM-HN-1 EC A24 NYNNFYRFL Monji, 2004 −0.42 223.7
KM-HN-1 EC A24 EYSKECLKEF Monji, 2004 −0.35 774.8
KM-HN-1 EC A24 EYLSLSDKI Monji, 2004 −1.34 502.4
LAGE-1 M A2 MLMAQEALAFL Aarnoudse, 1999 1.01 11.7
LAGE-1 M A2 SLLMWITQC Rimoldi, 2000 −1.33 390.1
LAGE-1 Various A31 LAAQERRVPR Wang, 1998 −0.63 113.5
LAGE-1 M B7 APRGVRMAV Slager, 2004 0.61 3.3
LAGE-1 BC A68 ELVRRILSR Sun, 2006 −0.31 93
LAGE-1 M B7 APRGVRMAV Slager, 2004 0.61 3.3
LRPAP1 Various A2 FLGPWAAS Marijt, 2019 −3.82 5299.3
LY6K Various A24 RYCNLEGPPI Suda, 2007 −1.37 519.5
MAGE-A1 M A1 EADPTGHSY Traversari, 1992 0.47 107.3
MAGE-A1 CC A2 KVLEYVIKV Pascolo, 2001 0.64 6.2
MAGE-A1 M A3 SLFRAVITK Chaux, 1999 0.14 16.2
MAGE-A1 BC A2 KVLEYVIKV Ottaviani, 2005 0.64 6.2
MAGE-A1 M A68 EVYDGREHSA Chaux, 1999 −2.92 7161.9
MAGE-A1 M B7 RVRFFFPSL Luiten, 2000 0.14 108.4
MAGE-A1 M B35 EADPTGHSY Luiten, 2000 1.24 16.1
MAGE-A1 M B37 REPVTKAEML Tanzarella, 1999 −1.49 3338.6
MAGE-A1 M B44 KEADPTGHSY Stroobant, 2012 0.59 144.9
MAGE-A1 M B53 DPARYEFLW Chaux, 1999 0.1 24.7
MAGE-A1 M B57 ITKKVADLVGF Corbière, 2004 −0.72 1775.4
MAGE-A1 M Cw2 SAFPTTINF Chaux, 1999 0.02 382.7
MAGE-A1 M Cw3 SAYGEPRKL Chaux, 1999 −0.43 219.7
MAGE-A1 MM Cw7 RVRFFFPSL Goodyear, 2011 −1.97 6638.8
MAGE-A1 M Cw16 SAYGEPRKL van der Bruggen, 1994 −0.75 525.8
MAGE-A10 M A2 GLYDGMEHL Huang, 1999 1.38 5.1
MAGE-A10 M B53 DPARYEFLW Chaux, 1999 0.1 24.7
MAGE-A12 m M A2 FLWGPRALV van der Bruggen, 1994 0.14 11.1
MAGE-A12 m BC Cw7 VRIGHLYIL Heidecker, 2000 −0.2 212.5
MAGE-A12 m M Cw7 VRIGHLYIL Panelli, 2000 −0.2 212.5
MAGE-A12 m M Cw7 EGDCAPEEK Breckpot, 2004 −3.8 43,575.9
MAGE-A2 M A2 YLQLVFGIEV Kawashima, 1998 −0.41 43.2
MAGE-A2 Various A24 EYLQLVFGI Tahara, 1999 −0.92 147.8
MAGE-A2 M Cw7 EGDCAPEEK Breckpot, 2004 −3.8 43,575.9
MAGE-A2 M B37 REPVTKAEML Tanzarella, 1999 −1.49 3338.6
MAGE-A3 M A2 KVAELVHFL Kawashima, 1998 0.98 11.2
MAGE-A3 M A1 EVDPIGHLY Gaugler, 1994 1.18 17.9
MAGE-A3 HNC A24 TFPDLESEF Oiso, 1999 −0.54 981.9
MAGE-A3 M B18 MEVDPIGHLY Bilsborough, 2002 0.94 46.1
MAGE-A3 M B35 EVDPIGHLY Schultz, 2001 0.51 74.6
MAGE-A3 M B37 REPVTKAEML Tanzarella, 1999 −1.49 3338.6
MAGE-A3 M B44 MEVDPIGHLY Herman, 1996 0.87 45.1
MAGE-A3 M B40 AELVHFLLL Schultz, 2002 0.6 19.9
MAGE-A3 M B52 WQYFFPVIF Russo, 2000 −0.52 1441.5
MAGE-A3 M Cw7 EGDCAPEEK Breckpot, 2004 −3.8 43,575.9
MAGE-A3 M A2 FLWGPRALV van der Bruggen, 1994 0.14 11.1
MAGE-A3 CRC A24 VAELVHFLL Miyagawa, 2006 −2.19 9428.9
MAGE-A4 Various A2 GVYDGREHTV Duffour, 1999 −1.47 971.6
MAGE-A4 M A1 EVDPASNTY Kobayashi, 2003 0.68 93.6
MAGE-A4 RCC A24 NYKRCFPVI Ottaviani, 2006 0 60.2
MAGE-A4 Various A24 NYKRCFPVI Miyahara, 2005 0 60.2
MAGE-A4 M B37 SESLKMIF Zhang, 2002 −1.68 11,466.1
MAGE-A6 M B35 EVDPIGHVY Benlalam, 2003 1.24 28.4
MAGE-A6 M Cw7 EGDCAPEEK Breckpot, 2004 −3.8 43,575.9
MAGE-A6 M Cw16 ISGGPRISY Vantomme, 2003 −0.11 628.4
#REF! M B37 REPVTKAEML Tanzarella, 1999 −1.49 3338.6
MAGE-A9 RCC A2 ALSVMGVYV Oehlrich, 2005 −0.32 31.4
MAGE-C1 MM A2 ILFGISLREV Anderson, 2011 0.2 11.2
MAGE-C1 MM A2 KVVEFLAML Anderson, 2011 0.84 19.7
MAGE-C2 M A2 ALKDVEERV Ma, 2004 −1.26 342.2
MAGE-C2 M B44 SESIKKKVL Godelaine, 2007 −1.48 3608.4
MAGE-C2 M A2 LLFGLALIEV Ma, 2004 0.52 9.9
MAGE-C2 M B57 ASSTLYLVF Ma, 2011 0.31 226.5
NA88-A M B13 QGQHFLQKV Moreau-Aubry, 2000 −3.07 16,389.9
NY-ESO-1/LAGE-2 M A2 SLLMWITQC Jäger, 1998 −1.33 390.1
NY-ESO-1/LAGE-2 M A2 SLLMWITQC Chen, 2000 −1.33 390.1
NY-ESO-1/LAGE-2 M A2 SLLMWITQC Valmori, 2000 −1.33 390.1
NY-ESO-1/LAGE-2 M A2 MLMAQEALAFL Aarnoudse, 1999 1.01 11.7
NY-ESO-1/LAGE-2 Various A24 YLAMPFATPME Eikawa, 2013 −4.36 32,521.3
NY-ESO-1/LAGE-2 Various A31 ASGPGGGAPR Wang, 1998 −1.4 498.5
NY-ESO-1/LAGE-2 Various A31 LAAQERRVPR Wang, 1998 −0.63 113.5
NY-ESO-1/LAGE-2 OC A68 TVSGNILTIR Matsuzaki, 2008 0.55 16.2
NY-ESO-1/LAGE-2 M B7 APRGPHGGAASGL Ebert, 2009 0.11 46.5
NY-ESO-1/LAGE-2 Various B35 MPFATPMEAEL Eikawa, 2013 −0.62 253.7
NY-ESO-1/LAGE-2 M B49 KEFTVSGNILTI Knights, 2009 −0.66 200.9
NY-ESO-1/LAGE-2 Various B52 FATPMEAEL Eikawa, 2013 −2.28 11,772.9
NY-ESO-1/LAGE-2 M Cw3 LAMPFATPM Gnjatic, 2000 0.48 2.6
NY-ESO-1/LAGE-2 M Cw6 ARGPESRLL Gnjatic, 2000 −1.38 2001
NY-ESO-1/LAGE-2 Various C12 FATPMEAELAR Eikawa, 2013 −2.63 16,931.9
NY-ESO-1/LAGE-2 M B51 MPFATPMEA Jäger, 2002 −2.09 704.3
SAGE Various A24 LYATVIHDI Miyahara, 2005 −0.41 43.1
Sp17 MM A1 ILDSSEEDK Chiriva-Internati, 2003 −3.5 18,212.4
SSX-2 M A2 KASEKIFYV Ayyoub, 2002 0.01 16.6
TAG-1 M A2 SLGWLFLLL Adair, 2008 0.17 52
TAG-2 M B8 LSRLSNRLL Adair, 2008 −1.68 4019.9
TRP2-INT2 M A68 EVISCKLIKR Lupetti, 1998 0.52 19
XAGE-1b/GAGED2a M A2 RQKKIRIQL Ohue, 2012 −2.19 19,497
Differentiation epitopes
CEA GIC A2 YLSGANLNL Tsang, 1995 0.71 18.8
CEA GIC A24 TYACFVSNL Nukaya, 1999 −0.05 60.1
CEA GIC A2 GVLVGVALI Alves, 2007 −1.64 637.3
CEA GIC A3 HLFGYSWYK Kawashima, 1999 0.17 7.9
CEA GIC A24 QYSWFVNGTF Nukaya, 1999 1.26 17
CEA GIC A2 IMIGVLVGV Kawashima, 1998 0.78 4.6
gp100/Pmel17 M A2 KTWGQYWQV Bakker, 1995 0.46 11.8
gp100/Pmel17 M A2 MLGTHTMEV Tsai, 1997 0.33 7.5
gp100/Pmel17 M A2 KTWGQYWQV Kawakami, 1995 0.46 11.8
gp100/Pmel17 M A2 ITDQVPFSV Kawakami, 1995 −1.2 211.5
gp100/Pmel17 M A2 YLEPGPVTA Cox, 1994 −1.09 172.7
gp100/Pmel17 M A2 VLYRYGSFSV Kawakami, 1995 0.12 12.3
gp100/Pmel17 M A2 LLDGTATLRL Kawakami, 1994 −0.51 347.7
gp100/Pmel17 M A2 RLMKQDFSV Kawakami, 1998 0.66 4.9
gp100/Pmel17 M A2 SLADTNSLAV Tsai, 1997 −0.32 47.7
gp100/Pmel17 M A3 LIYRRRLMK Kawakami, 1998 0.53 6.1
gp100/Pmel17 M A2 RLPRIFCSC Kawakami, 1998 −2.06 1491.6
gp100/Pmel17 M A3 IALNFPGSQK Kawashima, 1998 −0.19 31
gp100/Pmel17 M A3 ALLAVGATK Skipper, 1996 −0.35 45.2
gp100/Pmel17 M A3 RSYVPLAHR Michaux, 2014 0.3 68
gp100/Pmel17 M A3 ALNFPGSQK Kawashima, 1998 −0.03 19
gp100/Pmel17 M A24 VYFFLPDHL Robbins, 1997 −0.05 77.6
gp100/Pmel17 M A11 ALNFPGSQK Kawashima, 1998 −0.67 46.7
gp100/Pmel17 M B7 SSPGCQPPA Lennerz, 2005 −3.78 23,794.9
gp100/Pmel17 M A68 HTMEVTVYHR Sensi, 2002 1.34 2.6
gp100/Pmel17 M A32 RTKQLYPEW Vigneron, 2004 −0.02 69
gp100/Pmel17 M B35 VPLDCVLYRY Benlalam, 2003 0.23 154.7
gp100/Pmel17 M B35 LPHSSSHWL Vigneron, 2005 −0.73 240.8
gp100/Pmel17 M Cw8 SNDGPTLI Castelli, 1999 −3.07 31,048.6
mammaglobin-A BC A3 PLLENVISK Jaramillo, 2002 −2.42 2471.7
Melan-A/MART-1 M A2 ILTVILGVL Castelli, 1995 −0.6 529.6
Melan-A/MART-1 M A2 AAGIGILTV Kawakami, 1994 −2.13 3674
Melan-A/MART-1 M Cw7 RNGYRALMDKS Larrieu, 2008 −4.73 42,544.4
Melan-A/MART-1 M B35 EAAGIGILTV Benlalam, 2003 −3.08 15,289.2
Melan-A/MART-1 M A2 SLSKILDTV Wang, 2006 0.04 15.1
NY-BR-1 BC A24 LYSACFWWL Touloukian, 2003 0.04 91.7
OA1 M A2 TLMSAMTNL Olson, 2010 0.89 15.3
PAP PC A2 ALDVYNGLL Olson, 2010 −0.66 240.5
PAP PC A2 FLFLLFFWL Olson, 2010 0.22 42.5
PAP PC A2 FLTPKKLQCV Correale, 1997 −0.84 126.4
PSA PC A2 VISNDVCAQV Correale, 1997 −1.21 359
PSA PC A2 VLHWDPETV Walton, 2006 −0.58 116.7
RAB38/NY-MEL-1 M A31 MSLQRQFLR Wang, 1996 0.77 5.4
TRP-1/gp75 M A2 SVYDFFVWL Parkhurst, 1998 0.87 21.6
TRP-2 M A2 TLDSQVMSL Noppen, 2000 0.26 52.5
TRP-2 M A31 LLGPGRPYR Wang, 1996 0.27 24.1
TRP-2 M Cw8 ANDPIFVVL Castelli, 1999 −1.2 2060.1
TRP-2 M A33 LLGPGRPYR Wang, 1998 −0.47 115.1
TRP-2 M A1 KCDICTDEY Kittlesen, 1998 −0.62 1397.5
tyrosinase M A1 SSDYVIPIGTY Kawakami, 1998 0.43 147.3
tyrosinase M A2 MLLAVLYCL Wölfel, 1994 1.21 8.3
tyrosinase M A2 CLLWSFQTSA Riley, 2001 −1.08 99.6
tyrosinase M A2 YMDGTMSQV Wölfel, 1994 0.47 5.7
tyrosinase M A2 YMDGTMSQV Skipper, 1996 0.47 5.7
tyrosinase M A24 IYMDGTADFSF Dalet, 2011 1.09 23.4
tyrosinase M A24 AFLPWHRLF Kang, 1995 0.85 44.6
tyrosinase M A26 QCSGNFMGF Lennerz, 2005 −1.83 14,811.2
tyrosinase M B35 LPSSADVEF Morel, 1999 1.82 4.1
tyrosinase M B35 TPRLPSSADVEF Benlalam, 2003 −1.07 2534.5
tyrosinase M B38 LHHAFVDSIF Lennerz, 2005 −0.48 620.8
tyrosinase M B44 SEIWRDIDF Brichard, 1996 −0.16 250.7

Germ-line antigens are expressed in many tumors but not in normal tissues. Differentiation antigens are also expressed in the normal tissue of origin of the malignancy. Total score and MHC IC50 were predicted using the IEDB proteasomal cleavage/TAP transport/MHC class I combined predictor tool for mutant and wild-type peptides. Each line corresponds to a peptide that is considered to be a tumor antigen that is recognized by T cells. For each antigenic peptide, evidence of natural processing and presentation and isolation of stable human T cell clones that recognize the peptide were required for inclusion in the table. The MHC I binding predictions were made on March 27, 2020 using the IEDB Analysis Resource Consensus Tool94 that combines predictions from ANN a.k.a. NetMHC,95–97 SMM,98 and Comblib.99

HLA, human leucocyte antigen; IEDB, immune epitope database; MHC, major histocompatibility complex; TAP, transporter associated with antigen processing.