Table 1.
Confirmation of RT-PCR Limit of Detection
| Target 1 (ORF1) | Target 2 (E-Gene) | |||||
|---|---|---|---|---|---|---|
| Dilution | Replicates Detected | Average CT Value | Standard Deviation | Replicates Detected | Average CT Value | Standard Deviation |
| 10–1 | 4/4 (100%) | 25.38 | 0.148 | 4/4 (100%) | 25.41 | 0.135 |
| 10–2 | 5/5 (100%) | 28.43 | 0.122 | 5/5 (100%) | 28.44 | 0.155 |
| 10–3 | 5/5 (100%) | 31.43 | 0.386 | 5/5 (100%) | 31.74 | 0.439 |
| 10–4 | 5/5 (100%) | 34.17 | 0.168 | 5/5 (100%) | 34.86 | 0.178 |
| 10–5 | 5/5 (100%) | 35.95 | 0.273 | 5/5 (100%) | 37.16 | 0.574 |
| 10–6 | 3/5 (60%) | 36.99 | 1.372 | 2/5 (40%) | 37.52 | 1.060 |
| 10–7 | 0/5 (0%) | — | — | 0/5 (0%) | — | — |
CT, cycle threshold; RT-PCR, reverse transcription polymerase chain reaction.