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. 2020 Jul 18;5(1):bpaa014. doi: 10.1093/biomethods/bpaa014

Table 1:

Sample Cq values

Sample N1 N2 NP
AKL-MU-1 20.3 20.0 27.4
AKL-MU-2 20.6 20.4 27.0
AKL-MU-3 25.7 25.4 28.2
AKL-MU-4 28.5 28.2 29.6
AKL-MU-5 31.2 30.5 33.4

qPCR was performed using the CDC Primer probe set (IDT, Cat No./ID: 10006606). N1 and N2 target the nucleocapsid phosphoprotein (N gene) of SARS-CoV2. NP targets the RNase P gene for detection of human nucleic acids and serves as a control for sample integrity.