Table 2.
Detection of SARS-CoV-2 RNA in wastewater samples collected from three aircraft and a cruise ship
| Sources (sampling dates) | Virus concentration methods used | Sample ID (volume concentrated) | RT-qPCR positive results (Cq) | RT-ddPCR results | ||||
|---|---|---|---|---|---|---|---|---|
| CDC N1 | CDC N2 | N_Sarbeco | E_Sarbeco | NIID_2019-nCoV N | CDC N1 | |||
| Aircraft 1 Los Angeles–Brisbane (26 April 2020) |
Adsorption–extraction | A1a-1 (100 ml) | – | – | – | +(36.3) | – | – |
| A1a-2 (100 ml) | – | – | +(38.7) | –– | – | – | ||
| Amicon® Ultra-15 | A1b-1 (50 ml) | – | – | – | +(37.7) | – | – | |
| A1b-2 (50 ml) | – | – | – | – | – | – | ||
| A1b-3 (50 ml) | – | – | – | +(39.0) | – | – | ||
| Aircraft 2 Hong Kong–Brisbane (07 May 2020) |
Adsorption–extraction | A2a-1 (100 ml) | – | – | – | – | – | – |
| A2a-2 (100 ml) | – | – | – | – | – | – | ||
| A2a-3 (100 ml) | – | – | – | – | – | – | ||
| Aircraft 3 New Delhi–Sydney (10 May 2020) |
Adsorption–extraction | A3a-1 (100 ml) | – | – | – | – | – | NT |
| A3a-2 (100 ml) | – | – | – | – | – | NT | ||
| Cruise ship-influent (23 April 2020) | Adsorption–extraction | CSI1a-1 (100 ml) | +(33.6) +(33.2) +(33.4) |
+(35.8) +(37.6) |
– | – | – | + |
| CSI1a-2 (100 ml) | +(35.0) +(34.5) +(34.4) |
+(38.6) +(39.0) +(41.3) |
– | – | +(42.1) | + | ||
| CSI1a-3 (100 ml) | + (32.5) +(33.1) +(32.1) |
+(38.2) +(37.9) +(37.9) |
– | +(37.3) +(37.3) |
+(36.9) +(37.0) +(35.1) |
+ | ||
| Amicon® Ultra-15 | CSI1b-1 (50 ml) | +(36.1) | – | – | – | +(35.7) | + | |
| CSI1b-2 (50 ml) | +(36.6) | – | – | – | +(36.3) | – | ||
| CSI1b-3 (50 ml) | +(36.8) | +(38.2) +(38.4) |
– | – | – | – | ||
| Cruise ship-effluent (23 April 2020) | Adsorption–extraction | CSE1a-1 (200 ml) | – | – | – | +(38.7) | – | – |
| CSE1a-2 (200 ml) | – | – | – | – | – | + | ||
| CSE1a-3 (200 ml) | – | – | – | +(37.8) | +(36.0) | – | ||
| Amicon® Ultra-15 | CSE1b-1 (100 ml) | – | – | – | – | – | + | |
| CSE1b-2 (100 ml) | – | – | – | – | – | – | ||
–, not detected; +, positive; NT, not tested due to unavailability of reagents.