Fig 7. Cdc5 recruitment to the CCAN influences centromeric transcription.

(A) SAFE analysis as in Fig 1B of the Cdc5 SPIs and the CDC5ΔC-AME1 suppressor data identified highly dense regions that correspond to shared functions (mitosis and transcription for Cdc5 SPIs and transcription and mRNA processing for suppressors of CDC5ΔC-AME1). (B) A cartoon depicting Cdc5 recruitment to the kinetochore COMA complex (CCAN) resulting in increased production of CEN RNAs. We hypothesize that Cdc5 recruitment in late S-phase may influence CEN transcription by inhibiting a repressor such as Cbf1 and/or promote an activator such as Spt4 (see text for further details).