REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-human/mouse BCL6 PerCP-Cy5.5	Biolegend	RRID:AB_2566191
Anti-human CD279 PE	Biolegend	RRID:AB_940483
Anti-human CD3 BV421	Biolegend	RRID:AB_2565849
Anti-human CD4 BV510	Biolegend	RRID:AB_2561377
Anti-human CD8 APC	Biolegend	RRID:AB_2075390
Anti-human CD185 BV711	Biolegend	RRID:AB_2629525
Anti-human CD278 BV650	Biolegend	RRID:AB_2749928
Anti-human CD45RA APC-Cy7	Biolegend	RRID:AB_10708419
Anti-human CD45RO PE-Cy7	Biolegend	RRID:AB_11203903
Anti-human FOXP3 AF488	Biolegend	RRID:AB_430882
Anti-human CD25 BV785	Biolegend	RRID:AB_11219197
Anti-human CD45 AF488	Biolegend	RRID:AB_493033
Anti-human CD20 BV650	Biolegend	RRID:AB_11218609
Anti-human CD27 BV785	Biolegend	RRID:AB_2562674
Anti-human CD38 APC-Cy7	Biolegend	RRID:AB_2562576
Anti-human CD138 BV421	Biolegend	RRID:AB_2562659
Anti-human IgG Fc APC	Biolegend	RRID:AB_11150590
Anti-human IgM PE-Cy7	Biolegend	RRID:AB_2566484
Anti-human IgD BV510	Biolegend	RRID:AB_2561386
Anti-human CD21 PerCP-Cy5.5	Biolegend	RRID:AB_2561543
Anti-human CD45 BV785	Biolegend	RRID:AB_2715888
Anti-human CD3 BV605	Biolegend	RRID:AB_11126166
Anti-human CD19 BV711	Biolegend	RRID:AB_2562062
Anti-human CD56 AF488	Biolegend	RRID:AB_2564093
Anti-human CD11b PerCP-Cy5.5	Biolegend	RRID:AB_10900072
Anti-human CD68 APC-Cy7	Biolegend	RRID:AB_2571964
Anti-human CD14 PE-Cy7	Biolegend	RRID:AB_830690
Anti-human CD1c APC	Biolegend	RRID:AB_10718511
Anti-human HLA-DR BV421	Biolegend	RRID:AB_10897449
Anti-human CD16 BV510	Biolegend	RRID:AB_2562085
Anti-human CD56 BV421	Biolegend	RRID:AB_10900228
Anti-human CD27 AF488	Biolegend	RRID:AB_2565545
Anti-human CD107a AF647	Biolegend	RRID:AB_1227506
Anti-human CD107a PE-Cy7	Biolegend	RRID:AB_11147955
Anti-human CD3 PE	Biolegend	RRID:AB_314044
Anti-human CD19 APC	Biolegend	RRID:AB_314242
Anti-human CD4 BV711	Biolegend	RRID:AB_11219404
Anti-human CXCR5 BV785	Biolegend	RRID:AB_2629527
Anti-human CXCR5 AF488	Biolegend	RRID:AB_2561894
Anti-human CD279 BB700	BD Biosciences	RRID:AB_2744348
Anti-human CD19 BV421	Biolegend	RRID:AB_10897802
Anti-human CD3 PE-Cy7	Biolegend	RRID:AB_439781
Anti-human CXCR5 BB700	BD Biosciences	RRID:AB_2739739
Anti-human CD127 PE-Cy7	Biolegend	RRID:AB_10899414
Anti-human PD-1 APC	Biolegend	RRID:AB_940475
Anti-human FOXP3 eF450	Thermo Fisher	RRID:AB_1834364
Anti-human CD274 PE	Biolegend	RRID:AB_940368
Anti-human CD274 APC	Biolegend	RRID:AB_2749927
Anti-human PD-L1	R&D Systems	RRID:AB_2073445
Normal goat IgG control Antibody	R&D Systems	RRID:AB_354267
Anti-human CD3	Bio X Cell	RRID:AB_1107632
Bacterial and Virus Strains
DH5α E. coli	NEB	Cat# C2987H
XL10 Gold E. coli	Agilent	Cat# 200315
Stbl3 E. coli	Invitrogen	Cat# C737303
StellarComp E. coli	Clontech	Cat# 636763
VSV-G pseudotyped lentiviral vector	Viral Vector Core, Cincinnati Children’s Hospital Medical Center	https://www.cincinnatichildrens.org/research/cores/translational-core-laboratory/viral-vector-core/team
Biological Samples
Human tonsillar tissue	Division of Pediatric Otolaryngology-Head and Neck Surgery (Cincinnati Children’s Hospital Medical Center)	https://www.cincinnatichildrens.org/research/divisions/o/otolaryngology
Human cord blood	Cell Manipulations Laboratory & Cell Processing Core (Cincinnati Children’s Hospital Medical Center)	https://www.cincinnatichildrens.org/research/cores/translational-core-laboratory/cell-manipulations-laboratoryhttps://www.cincinnatichildrens.org/research/cores/translational-core-laboratory/cell-processing-core
Leukocyte reduction filters (source of human PBMC)	University of Cincinnati Hoxworth Blood Center	https://hoxworth.org/
Chemicals, Peptides, and Recombinant Proteins
Collagenase D	Roche	Cat# 11088793001
Recombinant Human IL-2	Peprotech	Cat# 200-02
DNase 1	Millipore Sigma	Cat# 11284932001
Ficoll-Paque PLUS	GE Healthcare	Cat# 17144002
BD Cytofix Fixation Buffer	BD Biosciences	Cat# 554655
BD Cytofix/Cytoperm Fixation and Permeabilization Solution	BD Biosciences	Cat# 554714
Perm/Wash Buffer	BD Biosciences	Cat# 554723
Ampicillin	GoldBio	Cat# A-301-25
Kanamycin	GoldBio	Cat# K-120
T4 DNA Ligase	New England BioLabs	Cat# M0202S
Human Fibronectin	Invitrogen	Cat# PHE0023
Protamine Sulfate	Fisher	Cat# ICN10275205
Blasticidin	Fisher	Cat# K515001
Puromycin	GIBCO	Cat# A1113803
rhPD-1-Fc	R&D Systems	Cat# 1086-PD-050
IgG1-Fc	R&D Systems	Cat# 110-HG-100
PMA	Sigma-Aldrich	Cat# P8139
Ionomycin	Sigma-Aldrich	Cat# I9657-1MG
Zombie UV Dye	Biolegend	Cat# 423107
SEB	EMD Millipore	Cat# 324798; CAS# 11100-45-1
Calcein AM Solution	Fisher	Cat# C34852
Propidium Iodide	Biolegend	Cat# 421301
Chromium-51	Perkin Elmer	Cat# NEZ030S002MC
CellTrace Violet	Invitrogen	Cat# C34557
Pristane	Sigma-Aldrich	Cat# P9622-10X1ML
Bulsulfan	Sigma-Aldrich	Cat# B2635-25G
UltraComp eBeads Compensation Beads	ThermoFisher	Cat# 01-2222-42
Critical Commercial Assays
DES Blasticidin Support Kit	Invitrogen	Cat# K515001
DES-Inducible Kit with pCoBlast	Invitrogen	Cat# K512001
GeneJET Gel Extraction kit	ThermoFisher	Cat# K0692
QIAprep Mini, Midi, Maxi Kits	QIAGEN	Cat# 27106, 12943, 12362
Rneasy Mini Kit	QIAGEN	Cat# 74104
iScript cDNA Synthesis Kit	Bio-Rad	Cat# 170-8891
PrimeTime Gene Expression Master Mix	IDT Technologies	Cat# 1055770
TaqMan Gene Expression Assay	ThermoFisher	Cat# 4331182
Easy Sep Human CD4+ T cell Isolation kit	StemCell Technologies	Cat# 17952
Easy Sep Human B cell Isolation kit	StemCell Technologies	Cat# 17954
Human IgG total ELISA Ready-SET-Go! Kit	eBioscience	Cat# 50-112-8669
Experimental Models: Cell Lines
Drosophila S2 cell line	ThermoFisher	Cat# R69007
HEK293T cell line	ATCC	RRID:CVCL_0063
NK-92 cell line	ATCC	RRID:CVCL_2142
Raji cell line	ATCC	RRID:CVCL_0511
Experimental Models: Organisms/Strains
Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CMV-IL3,CSF2,KITLG)1Eav/MloySzJ	Wunderlich et al., 201050	RRID:IMSR_JAX:013062
Oligonucleotides
Primer/probe sets for CAR expression qPCR	This paper	Table S1
Sequencing primers PD-L1 CAR plasmid	This paper	Table S1
Sequencing primers PD-1 pAc/V5-His plasmid	This paper	Table S1
Sequencing primers PD-1 PB513 plasmid	This paper	Table S1
Recombinant DNA
Human PDL1-CD28-CD3ζ chimeric antigen receptor	This paper	n/a
Human PD-1 protein sequence	Uniprot (a.a. 1-288)	n/a
pLVX-IRES-ZsGreen1 Vector	Clontech	Cat# 632187
pUC57-Kan plasmid vector	GeneWiz	n/a
pAc5.1/V5-His A Plasmid Vector	Invitrogen	Cat# V411020
PiggyBac Transposon, Cloning and Expression Vector	System Biosciences	Cat# PB513B-1
Super PiggyBac Transposase Expression Vector	System Biosciences	Cat# PB210PA-1
pcDNA3.1/His/lacZ	Invitrogen	Cat# V38520
pAc5.1/V5-His A	Invitrogen	Cat# V411020
Software and Algorithms
Sony SH800S Cell Sorter acquisition software	Sony Biotechnologies	https://www.sonybiotechnology.com/us/instruments/sh800s-cell-sorter/software/
Snapgene	GSL Biotech	https://www.snapgene.com/
Molecular Biology tool	Benchling	https://www.benchling.com/
“Codon Optimization Tool”	IDT Technologies	https://www.idtdna.com/pages/tools
NIS-Elements Imaging Software	Nikon	https://www.microscope.healthcare.nikon.com/products/software
FACSDiva	BD Biosciences	https://www.bdbiosciences.com/en-us/instruments/research-instruments/research-software/flow-cytometry-acquisition/facsdiva-software
FlowJo v.10 Software	TreeStar Inc.	https://www.flowjo.com/
Prism 7	GraphPad	https://www.graphpad.com/scientific-software/prism/
Nonlinear Regression (curve fit)	GraphPad Prism	https://www.graphpad.com/guides/prism/7/curve-fitting/index.htm?REG_DR_stim_variable_2.htm.