Figure 6. Tregs from the periweaning colon can rescue oral tolerance and immune skewing in desynchronized pups.

(A and B) A total of 5 × 10⁴ Foxp3⁺RORγt⁺ Tregs isolated from DOL21 colon LP or 5 × 10⁴ Foxp3⁺RORγt^– Tregs isolated from DOL21 spleen, MLN, and colon LP were transferred into DOL56 cross-fostered mice as described in Figure 5. (A) Serum IL-13 concentrations measured weekly after transfer. (B) On DOL98, mice were given Ova in drinking water for 1 week, immunized with Ova, and challenged with Ova in the footpad. The increase in footpad thickness was measured 24 hours later. Data are presented as the mean ± SEM, *P < 0.05. A and B are representative of 2 independent experiments with 5 or more mice in each group; offspring from 3 litters per condition were used in A and B. Significance was calculated using 2-way ANOVA with Dunnett’s post hoc test in A and 1-way ANOVA with a Dunnett’s post hoc test in B.