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. 2020 Aug 6;5(15):e138290. doi: 10.1172/jci.insight.138290

Figure 5. EPHA2 phosphorylation in PDAC cells conveys sensitivity to the inhibitor ALW-II-27.

Figure 5

(A) Differential expression of EPHA2 between bulk (n = 16) and isolated epithelial compartments (n = 15) identified EPHA2 as specifically but heterogeneously expressed in the tumor compartment (limma test, corrected for multiple testing, P = 0.05) (mean ± SD). (B) Reduction of EPHA2 expression by shRNA in Capan-2 cells significantly reduced proliferation rate (P < 0.001, unpaired 2-way t test) (mean ± SEM) (n = 7). (C) Dose-response curves of cell lines treated with ALW-II-27. (D) Western blot analysis of cells after treatment with ALW-II-27 shows reduction of EPHA2 expression and phosphorylation of activation site pY-588. See complete unedited blots in the supplemental material. (E) Transwell migration of Capan-2 cells was reduced upon pretreatment with 1 μM ALW-II-27, irrespectively of attractant (EGF, P = 0.0005, FCS P = 0.0029, 2-tailed t test with Welch’s correction) (mean ± SEM) (n = 3).