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. 2020 Aug 6;5(15):e136706. doi: 10.1172/jci.insight.136706

Figure 1. ERM upregulates DEL-1 mRNA and protein levels.

Figure 1

(A) DEL1 mRNA transcription was analyzed by quantitative PCR (qPCR) in HMVECs incubated for the indicated time periods with or without ERM (10 μg/mL), JSM (10 μg/mL), or PC (10 μg/mL). Data were normalized against GAPDH mRNA and expressed as fold induction relative to treatment with ethanol (vehicle control), which was assigned an average value of 1. (B) DEL-1 protein levels in the cell culture supernatants after 6-hour incubation were measured by ELISA. (C) Del1 mRNA transcription in the lung tissue was analyzed by qPCR 24 hours after i.p. injection of ethanol (vehicle control), ERM (20 mg/kg), JSM (20 mg/kg), or PC (20 mg/kg). Data were normalized against Gapdh mRNA and expressed as fold induction relative to treatment with ethanol (vehicle control), which was assigned an average value of 1. Data are presented as the mean ± SD; (A and B: n = 6 sets of HMVECs cultures; and C: n = 6 mice/group); (A) 2-way ANOVA followed by Holm-Šidák multiple comparisons test; (B and C) 1-way ANOVA followed by Tukey’s multiple comparisons test; *P < 0.01, ***P < 0.0001 between indicated groups.