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. 2020 Aug 20;5(16):e138729. doi: 10.1172/jci.insight.138729

Figure 5. CD28 costimulation increases glycolysis and glucose transporters.

Figure 5

(A) Glycolytic stress test results showing representative ECAR and OCR (±SEM) normalized to cell count using Cytation 5 (BioTek). CD8+ RCC TIL glycolysis following IL-7 (gray), CD3 alone (black), CD3 with CD28 costimulation (red). n = 5. *P < 0.05, ***P < 0.001 by 1-way ANOVA with Tukey’s post hoc test. (B) Flow cytometry analysis showing MFI of GLUT1 and GLUT3 normalized to IL-7 and comparing CD3 with CD28 costimulation (red). n ≥ 9. **P < 0.01 by 1-way ANOVA with Tukey’s post hoc test.