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. 2020 Aug 20;5(16):e133868. doi: 10.1172/jci.insight.133868

Figure 2. Metformin treatment in T2DM subjects is associated with decreased NPY-ir in the IFN, independent of additional insulin treatment.

Figure 2

(A–K) NPY-ir neurons in the IFN of a CTRL subject (A), a T2DM subject with no insulin no metformin (B), with metformin and insulin (C), or with insulin (D) (higher magnification of the areas framed in A–D are shown in E–H, respectively). (I–K) Comparison between CTRL and T2DM subjects on: NPY-ir neuronal number (I), NPY-ir soma size (J), and the relative area covered by NPY-ir (K). (L–N) The antidiabetic treatment-associated effect on NPY-ir neuronal number (L), NPY-ir soma size (M), and the relative area covered by NPY-ir (N). 3V, third ventricle. Scale bars: 500 μm (D), 50 μm (H). Data represent mean ± SEM. Significance is calculated using 2-way ANOVA (L–N). All P values were corrected for multiple testing using the Benjamini-Hochberg criterion. *P < 0.05, ***P < 0.001.