Figure 10. In vitro studies with human CD34⁺ cells.

(A) Schematic of the experiment: CD34⁺ cells were transduced with HDAd-long-LCR + HD-SB or HDAd-short-LCR + HDAd-SB and subjected to erythroid differentiation (ED). In vitro selection with O⁶BG-BCNU was started at day 5 of ED. At day 18 cells were analyzed by flow cytometry (B) and HPLC (C). (D) VCN at day 18. Statistical analyses were performed using 2-way ANOVA. *P < 0.05; **P < 0.0001.