Skip to main content
. 2020 Aug 28;6(35):eaba4996. doi: 10.1126/sciadv.aba4996

Fig. 1. Overall structure of Cx31.3 hemichannel with entrance-covering NTHs.

Fig. 1

(A and B) The overall structure of Cx31.3 hemichannel. Single-particle reconstruction map (A) and ribbon representations (B) are shown in the same orientations. Six connexins in the hemichannel are shown in different colors, and NTHs are in magenta color in (B). Yellow spheres indicate sulfur atoms in C2. (C) Comparison of NTH conformation in Cx31.3 hemichannel with those of Cx26 and Cx46 GJIChs. Only two interfacing connexins in a hexameric channel are shown for clarity. The accession codes for Cx26 and Cx46 GJICh structures are 2ZW3 and 6MHQ, respectively. NTHs are shown in magenta color. (D) Ribbon presentation of a single Cx31.3 connexin. NTH, TM helices, and ECLs are shown in different colors and are labeled. Cytoplasmic loop (CL) and C-terminal loop (CTL) are represented as gray dashed lines. (E) Intramolecular hydrophobic interactions between NTH and TM2. (F) The solvent-accessible pore radius through Cx31.3 hemichannel pore (gray) is compared with those through Cx26 (orange) and Cx46 (green) GJICh pores. Locations of C2 and the acidic band of Cx31.3 are indicated using an arrow and an asterisk.