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. 2020 Aug 28;6(35):eabc3646. doi: 10.1126/sciadv.abc3646

Fig. 1. Design, optimization, and characterization of PS3D1@DMXAA nanoparticles.

Fig. 1

(A) Schematic illustration of triblock copolymer PEG-b-PSN38-b-PDEA structure and preparation of DMXAA-loaded nanoparticles driven by electrostatic interactions, and the proposed mechanism of redox-triggered SN38 release from nanoparticles. (B) Characterization of self-assembly behaviors of copolymers and DMXAA EE. (C) Representative snapshots of PSN38-b-PDEA with DMXAA after MD simulation. PEG content was not included in the molecular dynamic simulation. Color scheme: blue, PSN38 block; green, PDEA block; red, DMXAA molecules. Solvent molecules have been removed for clarity. (D) Number of polymer clusters formed during the MD simulation. (E) DMXAA release profiles in nanoparticles immersed in NaCl solution (n = 3, mean ± SD). (F) Transmission electron microscopy image of PS3D1@DMXAA nanoparticles. (G) Variation of hydrodynamic particle diameter change recorded for self-assembly of PS3D1 with various amounts of DMXAA. (H) SN38 and DMXAA release profiles in PBS with/without DTT (D = DMXAA, S = SN38, n = 3, mean ± SD).