Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (duck) | DHBV16 | GenBank | K01834 | duck hepatitis B virus |
| Strain, strain background (Escherichia coli) | BL21Star (DE3) CodonPlus RIL | Thermofisher and Agilent |
C601003 #230240 |
BL21Star (DE3) transformed with RIL CodonPlus plasmid |
| Cell line (Gallus gallus) | LMH | ATCC | CRL-2117 | chicken hepatocellular carcinoma |
| Recombinant DNA reagent | pCD16 | Nassal lab Dallmeier et al., 2008 | CMV promoter controlled DHBV16 vector | |
| Recombinant DNA reagent | pAAV-DHBc | This paper | pAAV-MCS Addgene #46954 | authentic DHBV16 C gene cloned in pAAV-MCS2 for eukaryotic expression (see Plasmid Constructs) |
| Recombinant DNA reagent | pAAV-DHBc_Opt | This paper | pAAV-MCS Addgene #46954 |
E. coli optimized DHBV C gene cloned in pAAV-MCS2 for (see Plasmid Constructs) eukaryotic expression |
| Recombinant DNA reagent | pRSF-DHBc_Opt | This paper Heger-Stevic et al., 2018a |
E. coli optimized DHBV C gene cloned in pRSF under T7 promoter for bacterial expression; (see Plasmid Constructs) |
|
| Antibody | anti-DHBc (rabbit poylclonal) |
Vorreiter et al., 2007 | serum 12/99 | raised against recombinant full-length DHBc WB (1:10,000) For immuno precipitation (IP): 50 µl Antiserum 12/99 are loaded onto 500 µl Protein A Sepharose beads (Gelbett); 30 µl loaded beads are used per IP |
| Antibody | Goat IgG anti-Rabbit IgG (Fc)-HRPO | Jackson Immuno Research | Dianova Cat. # 111-035-046 | WB (1:10,000) |
| Chemical compound, drug | TransIT-LT1 | Mirus Bio | VWR Cat. # 10767–118 | IF(1:500) |
| Software, algorithm | Relion 3 | Zivanov et al., 2018 | ||
| Software, algorithm | MotionCor2 | Zheng et al., 2017 | ||
| Software, algorithm | Ctffind4 | Rohou and Grigorieff, 2015 | ||
| Software, algorithm | Chimera | Yang et al., 2012 |