. 2020 Aug 14;9:e57277. doi: 10.7554/eLife.57277

Table 1. Summary of image reconstruction and model building.

Sample prepared @	DHBc 2 weeks	DHBc 10 months	DHBcR124EΔ 2 weeks	DHBc+FkpA 2 weeks		DHBcR124E 3 months
Magnification	75,000
Acquisition mode	integrating mode	counting mode	Counting mode	Counting mode	Integrating mode	Integrating mode
Total exposure	39 e^-/Å²	77 e^-/Å²	60 e^-/Å²	60 e^-/Å²	40 e^-/Å²	40 e^-/Å²
Exposure time	3.5 s	75 s	54 s	56.6 s	2.4 s	2.3 s
Pixel size	1.0635 Å
Beam diameter	1 µm
C2 aperture	70 µm
Objective aperture	100 µm
Spot size	5	9	9	9	5	5
Number of movies per hole	2	3	2	3	3	2
Number of fractions	20	47	40	40	40	20
Number of movies	3541	2473	1496	2425	4339	1226
Defocus range	700–2200 nm	500–2100 nm	500–1300 nm	500–1600 nm	600–1900 nm	500–2000 nm
Acquisition software	EPU
Frame alignment and dose weighting	MotionCor2
CTF estimation	CtfFind4.1
Processing software	RELION3
Number of selected capsids	91,445	89,241	74,760	129,522	161,596	35,820
Capsids in final map	41,117	20,251	44,498	24,854	44,374	23,384
Resolution capsid	6.1 Å	3.7 Å	3.0 Å	4.2 Å	4.0 Å	6.0 Å
EMDB		10800	10803		10801	10802
extracted asymmetric units	2,428,920	1,215,060	2,669,880	1,210,560	2,662,440	n/a
Extracted units in final map	561,731	919,612	516,257	n/a	383,200	n/a
Resolution asymmetric unit	5.4 Å	3.0 Å	3.0 Å	n/a	3.9 Å	n/a
Model building, refinement and validation	-	Coot, Phenix, Chimera	Coot, Phenix, Chimera	-	-	-