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. 2020 Aug 28;11:4320. doi: 10.1038/s41467-020-18104-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Enhanced tubular epithelial expression of CFTR and TMEM16A in kidneys from Pkd1^−/− mice that was abolished in kidneys from Pkd1^−/−/T16a^−/− double-knockout mice. Both, CFTR and TMEM16A were colocalized in the apical membrane of the cyst epithelium. (blue; DAPI). b–d Expression of TMEM16A (^#P < 0.0001) and CFTR (^#P < 0.0001) were enhanced (n = 25 independent images over 5 animals) in Pkd1^−/− mice. Significant colocalization of TMEM16A and CFTR was detected (^#P = 0.0006) using colocalization finder algorithm (ImageJ, V.1.49 by Laummonerie and Mutterer). Expression of CFTR (^§P = 0.0006) and TMEM16A (^§P = 0.0006), as well as colocalization (^§P = 0.0001) were lower in Pkd1^−/−/T16a^−/− mice. e, f ki-67 staining was enhanced in tubular epithelial cells from Pkd1^+/+ (^#P < 0.0001) animals but was lower in cells from Pkd1^−/−/T16a^−/− kidneys (^§P < 0.0001) (n = 25 independent images from 5 animals). Mean and error bars indicating ±SEM. ^#§one-way ANOVA and Tukey’s post-hoc test comparing Pkd1^+/+ with Pkd1^−/− and Pkd1^−/− with Pkd1^−/−/T16a^−/−, respectively. Source data are provided as a Source Data file.