Table 2.

Primers used for generation of the MDCK-PKD1^−/− cell lines.

Guide primers
Exon 1 fw	5′-CACCGTGCTCCGGGCATTGGACGTT
Exon 1 rev	5′-AAACAACGTCCAATGCCCGGAGCAC
Cloning primers
Exon 1 cloning fw	5′-GCATGGTACCCAGAAAGGGAATGGCGCAG
Exon 1 cloning rev	5′-GCTAGCTAGCGCTTGGCTAATGACACCCAC
Off-target primers
Chrom 34 fw	5′-ACGTAAGCGGCAAGAGTCAA
Chrom 34 rev	5′-CTGCCTTCATCACTCCCAGG
Chrom 3 fw	5‘-AATTGGCTGCTGTGCAAAGC
Chrom 3 rev	5′-CAGCCTCTCCCCATAGCAAG