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. 2020 Aug 28;11:4343. doi: 10.1038/s41467-020-18208-y

Fig. 2. Oscar-−/− mice exhibit reduced OA pathogenesis.

Fig. 2

a–d Experimental OA was examined by safranin-O staining (a) and scoring of OA parameters, including articular cartilage destruction (OARSI grade) (b), SBP thickness (c), and ratio of hyaline cartilage (HC) to the calcified cartilage (CC) (d) in WT and Oscar−/− mice. Oscar−/− and WT mice underwent sham or DMM surgery for 8 weeks. Scale bar = 50 μm. e Representative 3D reconstructed micro-CT images and quantitative analysis of mouse tibia subchondral bone plates at 8 weeks post DMM surgery compared with that of the sham control. Scale bars = 1000 μm. Error bars represent mean ± S.E.M. of n = 10 mice (b–e). f, g qRT-PCR analysis of articular cartilage tissue of WT and Oscar−/− mice subjected to sham or DMM surgery with error bars representing mean ± S.E.M. of n = 10 mice. h, i IHC analyses of MMP3, MMP13 and ADAMTS5 (h), aggrecan and COL2A1 (i) in OA articular cartilage from DMM surgery mice compared with sham-operated mice (n = 10). Scale bar = 50 μm. Two-way ANOVA was performed followed by Sidak’s Multiple Comparison’s test, with p values indicated in figure.