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. 2020 Jul 23;47(8):5911–5925. doi: 10.1007/s11033-020-05662-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Capillary-like structure formation in Matrigel assay by HEPC-CB.1, HSkMEC.2 and HUVEC cells. A. The images show the tube formation by HEPC-CB.1 as compared to HSkMEC.2 and HUVEC cell lines, magnification ×40. B. Analysis of the results shown in A. C. Differentiation of HEPC-CB.1 cells leads to an increase in the efficiency of capillary-like structure formation. The results were obtained after 6 h of the test using the microscope Axiovert 200 M and AxioVision and ImageJ software. The values in the graphs represent the mean mesh size, number of nodes and total length ± SD of the representative experiment, determined on the basis of three independent measurements. P values were determined by t-test. *Indicates statistically significant differences (p < 0.05): in B. between HSkMEC.2 and HUVEC as compared to HEPC-CB.1 cells and in C. between differentiated and control HEPC-CB.1 cells