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. 2020 Aug 19;12:7451–7463. doi: 10.2147/CMAR.S252521

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© 2020 Hu et al.

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MiR-506-3p bound to STAT2 to modulate the development of NB. SK-N-SH and LAN-6 cells were transfected with miR-506-3p, miR-NC, miR-506-3p+pcDNA-STAT2 or miR-506-3p+pcDNA-NC. (A and B) Cell proliferation was monitored at different time points using MTT assay. (C and D) Colony formation assay was performed at 48 h post-transfection. (E and F) Cell cycle was assessed by flow cytometry assay at 48 h post-transfection. (G and H) The levels of CDK1 and Cyclin D1 at 48 h post-transfection were quantified by Western blot. (I–K) Glucose production, lactate production and ATP level were checked at 48 h post-transfection to assess glycolysis using the corresponding kit. *P < 0.05.