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. 2020 Aug 20;12:7487–7499. doi: 10.2147/CMAR.S257441

Figure 4.

Figure 4

Circ-ZNF609 modulates the malignant potential of PCa cells through sponging miR-501-3p. (A–J) DU145 and VCaP cells were transfected with si-NC (300 nM), si-circ-ZNF609 (300 nM), si-circ-ZNF609 (300 nM) + anti-miR-NC (400 nM) or si-circ-ZNF609 (300 nM) + anti-miR-501-3p (400 nM). (A) The expression of miR-501-3p was detected in PCa cells by qRT-PCR after transfection for 48 h. (B and C) Cell viability of PCa cells was analyzed using MTT assay after transfection for 72 h. (D) Flow cytometry was used to analyze the apoptosis rate of transfected PCa cells after transfection for 72 h. (E and F) The migration and invasion capacities of PCa cells were analyzed through transwell assays after transfection for 24 h. (G and H) The radioresistance of PCa cells was assessed through measuring survival fraction using colony formation assay. (I and J) The influence of circ-ZNF609 and miR-501-3p in the glycolysis of PCa cells was evaluated using fluorescence-based glucose and lactate assay kits. (K and L) PCa cells were treated with anti-miR-NC, anti-miR-501-3p or anti-miR-501-3p + 2-DG, and colony formation assay was used to analyze the radioresistance of DU145 and VCaP cells with various doses of irradiation. *P<0.05.