Table 5.

The eradication activity of the synergistic combinations against biofilm associated with P. aeruginosa strains

Tested strains	Plant extracts alone and combined with antibiotic at MIC level
	POM	ROS	POM/ROS	POM/ROS/PRL	POM/ROS/CDZ	POM/ROS/IPM	POM/ROS/CN	POM/ROS/LEV
	Biofilm eradication mean %1 ± SD
PS-1	41.20 ± 0.5a	54.14 ± 0.3e	82.35 ± 1.3f	91.52 ± 0.6h	99.60 ± 0.04 i	93.33 ± 1.3h	94.32 ± 0.60 h	93.70 ± 1.3 h
PS-2	62.51 ± 0.8b	55.26 ± 1.8e	84.60 ± 1.5f	93.80 ± 2.1h	98.70 ± 1.70 i	98.10 ± 2.1 i	95.80 ± 2.10 h	94.80 ± 1.4 h
PS-4	60.20 ± 1.1b	70.70 ± 0.5c	76.5 ± 1.0g	95.50 ± 0.7h	98.60 ± 1.70 i	96.30 ± 1.3 h	97.70 ± 0.70 i	97.10 ± 1.8i
PS-5	71.5 ± 1.00c	73.70 ± 1.4c	84.10 ± 1.6f	91.30 ± 1.7g	97.30 ± 2.10 i	95.10 ± 0.1 h	98.30 ± 0.50 i	97.40 ± 1.9 i
PS-19	48.40 ± 2.1d	53.10 ± 0.2e	85.40 ± 1.5f	90.00 ± 1.3h	98.30 ± 1.30 i	98.20 ± 1.7i	94.00 ± 0.40 h	98.30 ± 1.1 i

1, the eradication % calculated from biofilm control (untreated). POM, Pomegranate (1.6 mg/ml); ROS, Rosemary (3.1 mg/ml); PRL, piperacillin (0.032 mg/ml); IPM, imipenem (0.004 mg/ml); CDZ, ceftazidime (0.002 mg/ml); CN, gentamicin (0.002 mg/ml); LEV, levofloxacin (0.002 mg/ml); SD, standard division. Statistical analysis was performed using two-way ANOVA and Tukey’s test (P < 0.05); different small letters represent the significant between means of biofilm eradication %. The most effective combination shown in bold letters