Table 1.

Amino acid sequence and DNA sequences of 6× His-Trx-PRW4

	Sequences	Number of amino acids/gene length (bp)
Amino acid sequences	KRHHHHHHMAIVKATDQSFSAETSEGVVLADFWAPWCGPCKMIAPVLEELDQEMGDKLKIVKIDVDENQETAGKYGVMSIPTLLVLKDGEVVETS0VGFKPKEALQELVNKHLDDDDKRFRRLRWKTRWRLKKI	131
DNA sequences	CTCGAGAAAAGACATCACCACCACCACCACATGGCTATCGTTAAAGCTACAGATCAATCTTTCTCTGCTGAAACATCTGAAGGCGTTGTTCTTGCTGATTTCTGGGCTCCTTGGTGCGGCCCTTGCAAAATGATCGCTCCTGTTCTTGAAGAACTTGATCAAGAAATGGGCGATAAACTTAAAATCGTTAAAATCGATGTTGATGAAAACCAAGAAACAGCTGGCAAATACGGCGTTATGTCTATCCCTACACTTCTTGTTCTTAAAGATGGCGAAGTTGTTGAAACATCTGTTGGCTTCAAACCTAAAGAAGCTCTTCAAGAACTTGTTAACAAACATCTTGATGATGATGATAAACGTTTTCGCCGATTACGGTGAAAACTTAGATGGAGGTTGAAGAAAATTTAATCTAGA	414

The underlined and bold regions are the XhoI and XbaI cleavage site; the underlined regions are the Kex2 cleavage site, enterokinase digestion sequences; the italic region is the 6× His-Trx tag sequence; the shaded region is the target peptide PRW4 sequence; the bold region is the stop codon