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. 2020 Aug 28;11:4323. doi: 10.1038/s41467-020-17953-4

Fig. 3. Rare endogenous Wnt-active MB cells from treatment-refractory MBs have reduced tumorigenic properties.

Fig. 3

a SU_MB002 cells transduced with a Wnt reporter show enhanced TCF reporter activity in TGP+ cells compared with TGP− cells (p = 0.0035) (n = 3, independent experiments). b Differential Axin2 (p = 0.0007), Bmi1 (p = 0.0005), and Sox2 (p = 0.0028) transcript levels in TGP+ and TGP− cells (n = 3, independent experiments, all samples normalized to GAPDH). c Proliferative (p = 0.0012) and d self-renewal capacity (p = 0.0031) of TGP+ cells are significantly reduced when compared with TGP− cells (n = 3, independent experiments). e Representative histology images illustrating reduced tumor burden in TGP+ (n = 10) compared with TGP− xenografts (n = 10). f TGP+ xenografts (n = 10, median survival 39.0 days) display a significant increase in overall survival when compared with TGP− xenografts (n = 10, median survival 29.0 days) (p = 0.000012). g TGP+ tumors revert to TGP− as deduced by IHC for GFP expression. h Treatment-refractory MB patients (n = 113) with high expression of the Wnt hallmark signature have an improved overall survivorship (median survival undetermined) when compared with those patients with low Wnt hallmark signature expression (median survival 54.0 months) (p = 0.0126). Xenograft H/E histology image scale bar = 5000 μm. IHC histology image scale bar = 50 μm. Panels (a–d) contain error bars expressed as mean ± standard error (mean) using two-tailed, unpaired Student’s t test. Panels (f, h) analyzed using log-rank (Mantel–Cox) test. ****p < 0.0001.