Fig. 1. TEV-mediated cohesin removal in the C. elegans germline.
a Example of a dissected wild-type C. elegans germline stained with DAPI indicating key meiotic events and the timing of appearance and disappearance of different cytological markers of pairing and recombination. The horizontal dotted line encloses 34 nuclei progressing from leptotene to late pachytene. Nuclei move horizontally along the germline at a rate of ~1 row per hour, as indicated by two vertical rows of nuclei enclosed by dotted lines. REC-8 and COH-3 localisation in the same germline is shown in Supplementary Fig. 1e. b Projections of nuclei at the indicated stages before and after (3.5 h) TEV injection demonstrating efficient and specific removal of REC-83XTEV::GFP and COH-33XTEV::mCherry. Data are representative of two independent experiments. Scale bar = 5 µm. See also Supplementary Fig. 1.