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. 2020 Aug 28;3:476. doi: 10.1038/s42003-020-01201-y

Fig. 4. CNOT3 is essential for β-cell maturation and identity.

Fig. 4

a qPCR analysis of progenitor cells/dedifferentiation markers, normalized to the Gapdh mRNA level, in control and Cnot3βKO islets (n = 4–6). b Immunoblot analysis of ALDH1A3 in islet lysates from 8-week-old control and Cnot3βKO mice. This blot is a representative of three different blots. c Band quantification of an immunoblot of ALDH1A3 (n = 3) in Fig. 4b. d qPCR analysis of β-cell-specific functional mRNAs expression categorized as β-cell-specific transcription factors, glycolytic pathway, insulin granule maturation, and insulin secretion mRNAs, normalized to the Gapdh mRNA level, in control and Cnot3βKO islets (n = 3–7). e Co-immunofluorescence staining of MAFA (green), GLUT2 (green), and insulin (magenta) in pancreatic sections from 8-week-old control and Cnot3βKO mice. A scale bar represents 25 µm. Representative results from four 8-week-old mice from each genotype are shown. f qPCR analysis of immature β-cell markers, normalized to the Gapdh mRNA level, in control and Cnot3βKO islets (n = 5–7). g Immunoblot analysis of MCT1 and LDHA in islet lysates from 8-week-old control and Cnot3βKO mice. This blot is a representative of three different blots. h Band quantification of immunoblot of MCT1 and LDHA (n = 3). Data are presented as mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001, two-tailed Student’s t test.