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. 2020 Aug 28;3:476. doi: 10.1038/s42003-020-01201-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a 3847 genes were differentially expressed (DE) in islets following CNOT3 depletion in β cells. The graph indicates the percentage distribution of genes differentially expressed (P < 0.05) in Cnot3βKO compared with control islets (n = 3, each sample was pooled from two mice). Multidimensional scaling (MDS) plot is presented in Supplementary Fig. 10. b GO analysis of mRNAs significantly upregulated (red) and downregulated (blue) in Cnot3βKO islets. Bar charts of GO terms (Biological Process) ranked by FDR (<0.05) are shown. Gene lists included in each GO term are summarized in Supplementary Data 1. c Volcano plot of DE genes indicating the percentage distribution of DE (P < 0.05) genes by more than twofold upregulation (red) or downregulation (blue). d Pearson correlation analysis between differentially expressed genes and proteins identified by RNA-seq analysis and MS analysis of control and Cnot3βKO islets. e Venn diagram showing the overlap of significantly upregulated (red) and stabilized (green) mRNAs in Cnot3βKO compared with control islets. f qPCR analysis of three top upregulated and stabilized mRNAs (Aldob, Slc5a10 and Wnt5b), normalized to the Gapdh mRNA level, in control and Cnot3βKO islets (n = 3–6). g Immunoblot analysis of ALDOB in islet lysates from 8-week-old control and Cnot3βKO mice (n = 3). This blot is a representative of three different blots. h Band quantification of immunoblot of ALDOB (n = 3). Data are presented as mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001, two-tailed Student’s t test.