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. 2020 Aug 28;10:14237. doi: 10.1038/s41598-020-71012-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Impacts of water availability on transcript abundance of C₄- and CAM-marker genes. (A-C) Relative abundance of CAM-specific transcripts: PPC1E1c (A), ALMT-12E.1 (B), DIC-1.1 (C). (D–F) Relative abundance of CAM-specific transcripts: PPC1E1a’ (D), NADME-2E.1 (E), ASPAT-1E1 (F). Mean relative expression was normalized against well-watered trituberculata samples, and mRNA levels were determined in samples harvested at dawn (A–C) or dusk (D–F). Data are means ± SE of at least three biological replicates, and different letters indicate statistically significant differences (p < 0.05) among the treatments for each subspecies.