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. 2020 Aug 28;10:14240. doi: 10.1038/s41598-020-71120-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cfap52 expression is not correlated with the presence of motile cilia. RT-PCR on cDNA synthesized from different mouse tissues and from the fibroblast cell line NIH3T3. (a) Amplification of exon 1 to exon 14 of Cfap52. The expected fragment size of 1871 bp is found in testis and ovary. (b) Nested PCR on the first RT-PCR products was performed to amplify parts of exon 4 to exon 6 of Cfap52. The expected fragment size of 255 bp is present in all probes. (c) Gapdh amplification as quality check. Control is the PCR reaction without template.