Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 28;10:14240. doi: 10.1038/s41598-020-71120-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

CFAP52 locates to the centrosome, the basal body, the intercellular bridge, and the spindle poles in NIH3T3 cells. (A) The full-length pCfap52::Egfp expression plasmid was transfected into NIH3T3 cells and detected by auto-fluorescence (green) (a). Immuno-decoration of the centrosome with the centrosomal marker γ-tubulin (red) revealed localization of CFAP52 in the centrosome (b–d). Bars are of 2 µm. (B) Endogenous expression of CFAP52 in NIH3T3 cells was detected by double immunostaining with anti-CFAP52 (green) and either anti-γ-tubulin (red) or anti-acetylated tubulin (red). γ-tubulin and acetylated tubulin decoration for detection of the centrosome, acetylated tubulin for decoration of the basal body, the spindle poles, the intercellular bridge, and the primary cilium. The endogenous CFAP52 (arrows) locates to the centrosome (a–d), the intercellular bridge (e–h), the basal body and its associated daughter centriole (i–l), and the spindle poles (m–p). Omitting anti-CFAP52 showed decoration of the centrosome by anti-acetylated tubulin staining (red) but no false CFAP52 staining (q–t). Nuclear counterstain with DAPI (blue). Bars are of 2 µm except for (e–h), which are of 5 µm.