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. 2020 Aug 28;10:14240. doi: 10.1038/s41598-020-71120-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Centrosomal location of EGFP-fusion proteins of CFAP52 isoforms 203 (CFAP52-203::EGFP) and the N-terminal end of isoform X1 (CFAP52-X1-Nterm::EGFP). Expression plasmids for the isoform 203, pCfap52-203::Egfp (a–h), and the N-terminal sequence of CFAP52-isoform-X1, pCfap52-X1-Nterm::Egfp (i–p) were transfected into NIH3T3 cells and detected by the EGFP auto-fluorescence (green, a, e, i, m). Decoration of the centrosomes by immunostaining for the centrosomal marker proteins either γ-tubulin (anti-gamma-tubulin, b, j) or Pericentrin (anti-Pericentrin, f, n) (both in red). Nuclear counterstain with DAPI (blue). Arrows pointing to the centrosomal location of the fusion proteins. Bars are of 2 µm (a–p).