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. 2020 Aug 28;20:417. doi: 10.1186/s12935-020-01503-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Validation of a Kras^G12D.Tgfbr2^−/− cell line. a H&E stain showing orthotopic tumor formation by line X911. Scale bar is 100 µm and the red line denotes the tumor border. b PCR showing the recombinant Kras^G12D allele (305 bp) and Tgfbr2 allele (220 bp) in X911 cells but not in CMT or LLC cells. c Western blot showing KRAS^G12D expression and TGFBR2 loss in X911 cells. The band at ~ 70 kD is nonspecific (NS)