Fig. 7.

Validation of an EML4-ALK rearranged cell line. a H&E stain showing orthotopic tumor formation by line Y143. Scale bar is 100 µm and the red line denotes the tumor border. b PCR of genomic DNA demonstrating the 190 bp EML4-ALK rearrangement. Positive control DNA was harvested directly from lung tumors induced by the Ad-EA virus while negative control DNA was harvested from normal mouse lung. c Crizotinib treatment reduces pAKT and pERK in Y143 cells. d Y143 growth can be inhibited in vitro by TAE684 with an IC50 of 22 nM