Fig. 3.
AP-1mutp infection of Kasumi-3 cells results in a significant reduction of AP-1 transcription factor binding to the MIE enhancer in the presence of reactivation stimuli. Kasumi-3 cells were infected (moi = 1.0) with the indicated viruses. At 7 dpi, half of each infected population was cultured for an additional 2 d with vehicle (DMSO; −TPA, blue bars) or TPA (+TPA, red bars). AP-1 transcription factor binding to the MIEP was quantified by ChIP using (A) α-fos or (B) α-jun antibodies. Coprecipitated MIEP was quantified by qPCR, and data are shown as fold-change relative to input. Each data point (circles) is the mean of three technical replicates. Error bars indicate SD of three biological replicates, and the statistical significance was calculated using two-way ANOVA followed by Tukey’s post hoc analysis. *P < 0.05, **P < 0.01, ***P < 0.001, and ns = not significant. Abbreviations: AP-1mutp (mutp), AP-1repp (repp).