Table 1.

Minimal inhibitory concentrations (MICs) of TET and SAR against various E. coli strains carrying indicated mutations in the 16S rRNA

E. coli strain	Mutation	MIC, µg/mL (fold change relative to WT)
		TET	SAR
SQ110∆TolC	WT	0.5	1.0
	16S: U1052G	0.125 (0.25)	0.5 (0.5)
SQ171∆TolC	WT	0.125	0.125
	G966U	0.5 (4)	2 (16)
	G1058C	0.5 (4)	1 (8)
	U1060A	0.5 (4)	4 (32)
	A1197U	2 (16)	2 (16)
	U1060A+A1197U	0.125 (1)	0.125 (1)

E. coli strain SQ110∆TolC carries only one rrn operon. E. coli strain SQ171∆TolC lacks all chromosomal rrn operons and, instead, carries pAM552 plasmid with a mutated rRNA operon.