Fig. 2.

In vitro phosphorylation rhythms of mutants at position 402 of KaiC. (A) Representative in vitro phosphorylation rhythms and periods (τ) of Y402A, Y402C, Y402L, Y402F, WT, and Y402W at 30 °C. (B) Periods of in vitro phosphorylation rhythms of Y402A, Y402C, Y402L, Y402F, WT, and Y402W, plotted against van der Waals volumes of residue 402. r, correlation coefficient. p, P value. (C) Effect of temperature on period lengths of the in vitro phosphorylation rhythms of Y402A, Y402C, Y402L, Y402F, WT, and Y402W. Symbols are as in A. Values are represented as means ± SD. The sample sizes (n) are shown in SI Appendix, Table S1.