Fig. 6.

Apilimod and Vacuolin-1 inhibit infection of VSV-eGFP-SARS-CoV-2. (A) Schematic of infectivity assay of VSV-eGFP, VSV-eGFP-ZEBOV, and VSV-eGFP-SARS-CoV-2 in MA104 cells. MA104 cells were pretreated for 1 h with the indicated concentration of Apilimod. Pretreated cells were inoculated with the indicated virus (MOI = 1) for 1 h at 37 °C. At 6 h postinfection cells were harvested, and the fraction of cell expressing eGFP cells was quantified by flow cytometry. (B) Quantification of the infectivity is shown with averages ± SEM from three independent experiments. Statistical significance was determined using a t test (*P ≤ 0.05; **P ≤ 0.01).