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. Author manuscript; available in PMC: 2020 Aug 29.
Published in final edited form as: Methods Mol Biol. 2017;1627:253–283. doi: 10.1007/978-1-4939-7113-8_18

Fig. 10.

Fig. 10

Recellularization of different compartments of decellularized human lung lobes. Excised segments of acellular human lung were selectively seeded through airways or vasculature with the use of a calcium alginate coating. (a) Identification of small airways and vessels, cannulation and securement with surgical clips for controlled cellular inoculation. (b) Human bronchial epithelial (HBE) and pulmonary endothelial colony-forming (CBF) cells were retained in their respective compartment after 24 h of culture (Reprinted with permission from Ref. 12)