Fig. 1.

Ablation of Dot1L results in CD44⁺CD62L⁺ memory-like CD8⁺ T cells. (A) Flow-cytometry analysis of CD8⁺ T cell subsets in the spleen based on CD44 and CD62L expression in WT and heterozygous and homozygous Dot1L-KO mice. Subsets are indicated in the Top Left. (B) Quantification of CD8⁺ T cell subsets in the spleen indicated in the representative plots in A. Data from three to five individual experiments with three to four mice per genotype per experiment, shown as mean ± SD. (C) Mean average (MA) plot of RNA-Seq data from FACS-sorted CD44⁺CD62L⁺ (T_CM) and CD44⁻CD62L⁺ (T_N) CD8⁺ T cells from four mice per indicated genotype. Naïve and memory signatures were defined based on differentially expressed genes (false discovery rate [FDR] < 0.01) between WT T_N and WT T_CM cells. (D) Percentage of CD49d⁺ cells in CD44⁺CD62L⁺ CD8⁺ T cells from unchallenged mice and in CD44⁺CD62L⁻ CD8⁺ T cells from WT mice challenged with L. monocytogenes for 7 d. Data are from one experiment with four mice per genotype, represented as mean ± SD. (E) Representative flow-cytometry plots of T-bet and Eomes expression in CD8⁺ T cells from the spleen. (F) Median fluorescence intensity (MFI) of T-bet and Eomes in CD44⁺CD62L⁺ CD8⁺ T cells from the spleen. Data are from one experiment with three mice per genotype, represented as mean ± SD.