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. 2020 Jul 27;130(9):4652–4662. doi: 10.1172/JCI135754

Figure 3. Human CD83 CAR T cells reduce alloreactivity.

Figure 3

Human T cells were cultured with allogeneic cytokine-matured moDCs at a DC/T cell ratio of 1:30 (i.e., 100,000 T cells and 3333 moDCs). CD83 CAR T cells were added at specific ratios to the moDCs (3:1 to 1:10, where the lowest amount of CAR T cells added was 333 cells). T cell proliferation was measured by Ki-67 expression at day +5. CAR T cells were gated out by their expression of GFP. Controls included T cells alone (i.e., no proliferation), mock-transduced T cells, and CD19 CAR T cells. These mock-transduced T cells did not express a CAR but were treated in an identical fashion as the transduced CD83 CAR T cells. The CD19 CAR T cells used an identical 41BB costimulation domain as the CD83 CAR T cells, but targeted an irrelevant antigen. One of 2 representative experiments is shown.