Figure 5. Human CD83-targeted CAR T cells significantly reduce CD83⁺ DCs.

NSG mice received 25 × 10⁶ human PBMCs plus 1 × 10⁶ CD83 CAR or mock-transduced T cells as described. Mice were humanely euthanized on day +21 and the spleens were harvested. (A) Representative contour plots show the frequency of human CD1c⁺ CD14^– DCs; CD1c⁺ CD83⁺ DCs; and CD1c⁺ MHC class II⁺ DCs in the mouse spleens at day +21. Graph shows the absolute number (mean ± SEM) of human (B) CD1c⁺ CD83⁺ DCs and (C) CD1c⁺ MHC class II⁺ DCs in the mouse spleens at day +21. Pooled data from 2 independent experiments, up to 6 mice per experimental arm. ANOVA (B and C). *P < 0.05; **P = 0.001–0.01.